Serum-Free Reduced Enriched modifications of Medium 199, formulated for growth and maintenance of primary cells.
SFRE Medium 199 is a modification of Medium 199 developed for growth and maintenance of primary baboon kidney (Bak) cells. Both the media were formulated by supplementing medium M199 with insulin, sodium pyruvate, zinc sulfate, and increasing arginine-HCl, cysteine, cystine, L-glutamine, L-glutamic acid, glycine, histidine, tyrosine, and glucose to maximally active nontoxic concentrations. SFRE 199-2 is additionally supplemented with galactose to avoid excessive accumulation of lactic acid and to maintain pH in the physiological range for prolonged maintenance of the cells.
SFRE Medium 199-1 and 199-2 formulations in dry powder format. Highly suited for serum-free primary cell culture.
All BioArtha classical powder media are validated, high-purity selection reagents for cell culture.